15N TROSY-selected ZZ-exchange

This experiment measures exchange between two or more molecular species that are in slow exchange on the NMR chemical shift time scale.

The experiment normally is performed using protein samples that are perdeuterated at carbon sites [U-D,U-15N] to reduce 1H-1H dipole interactions.


Y. Li and A. G. Palmer, TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins, J. Biomol. NMR 45, 357–360, (2009).

Pulse Sequence and Parameter Set