Columbia University GG6270 Instructors: Ann McDermott and Arthur Palmer Location: New York Structural Biology Center Times: TBD First class: January 19, 2018 Textbook: Protein NMR Spectroscopy: Principles and Practice Cavanagh, Fairbrother, Palmer, Rance, Skelton Academic Press, 2007 ISBN 978-0-12-164491-8 Optional: Multidimensional Solid-State NMR and Polymers Schmidt-Rohr and Spiess Academic Press, 1996 ISBN 0-12-626630-1
Multi-dimensional NMR spectroscopy has become a powerful technique for investigation of the structural and dynamical properties of biological macromolecules in solution and solid state. This course will present a detailed survey of the principles of modern techniques in NMR spectroscopy as applied to biological macromolecules, including proteins, nucleic acids and carbohydrates. Both theoretical and experimental aspects of NMR spectroscopy, structure determination and investigation of rate processes will be discussed. The theoretical description of NMR spectroscopy will rely heavily on a rigorous, but simplified, quantum mechanical treatment known as the product operator formalism.
The format of the course consists of lectures by the instructors and readings from the original literature. Problem sets will be based on the lectures and reading assignments. Grading will be based on a mid-term examination, and a final paper. Paper topics will be chosen by the students in consultation with the instructors.
Students and postdoctoral scientists already performing NMR research are invited to audit any or all parts of the course. Additional Reading List
A. Abragam “Principles of Nuclear Magnetism” Claredon Press (1961). R. R. Ernst, G Bodenhausen and A Wokaun “Principles of Nuclear Magnetic Resonance in One and Two Dimensions” Oxford Science Publications (1987). U. Haeberlen“High Resolution NMR in Solids: Selective Averaging” (1976), also published in “Advances in Magnetic Resonance, Supplement 1”. P. J. Hore “Nuclear Magnetic Resonance” Oxford Chemistry Primers (1995) P. J. Hore, J. A. Jones, and S. Wimperis “NMR : The Toolkit” Oxford Chemistry Primers (2000) G. C. K Roberts (Ed.) “NMR of Macromolecules: A practical approach” IRL Press (1993). K. Wüthrich “NMR of Proteins and Nucleic Acids” Wiley (1986).