3D DCP CORD

This is NYSBC standard data set and pulse program for DCP CORD 3D The pulse program DCP_CORD_3D.nysbc, ramp shape file tancn_100_SCALE and CPD files are located in the dataset directory.

Citation

Broadband Homonuclear Correlation Spectroscopy Driven by Combined R2(n)(v) Sequences Under Fast Magic Angle Spinning for NMR Structural Analysis of Organic and Biological Solids, Guangjin Hou 1, Si Yan, Julien Trébosc, Jean-Paul Amoureux, Tatyana Polenova, JMR 2013 (232) pp.18-30) [https://pubmed.ncbi.nlm.nih.gov/23685715/[(https://pubmed.ncbi.nlm.nih.gov/23685715/)

13C-1H dipolar assisted rotary resonance in magic angle spinning NMR, Takegoshi, K, Nakamura S and Terao, T Chem Phys Lett 2001 (344) pp 631-637 [https://www.sciencedirect.com/science/article/abs/pii/S0009261401007916[(https://www.sciencedirect.com/science/article/abs/pii/S0009261401007916)

Sample heating

To minimized heating of conductive samples, we recommend using an Efree/lowE probe. However, a combination of frictional heating from spinning and RF heating on long pulses on both channel may cause substantial sample heating and temperature gradient across the sample even in an Efree probe. The heating may damage the sample. The heating and temperature gradient across the rotor may substantially compromise the precision of measurements and data fitting.

Citation

https://www.ncbi.nlm.nih.gov/pubmed/10868566?dopt=Abstract Heating of samples induced by fast magic-angle spinning, Brus, J., Solid State Nucl. Magn. Reson. 16, 151–160 (2000).

https://onlinelibrary.wiley.com/doi/abs/10.1002/mrc.4450 Heating and temperature gradients of lipid bilayer samples induced by RF irradiation in MAS solid-state NMR experiments, Wang, J., Zhang, Z., Zhao, W., Wang, L. & Yang, J., Magn. Reson. Chem. 54, 753–759 (2016).

https://www.biorxiv.org/content/10.1101/566729v1.full TmDOTP : An NMR- based Thermometer for Magic Angle Spinning NMR Experiments Dongyu Zhang, Boris Itin, Ann E. McDermott; preprinted

Probe and power limitations

Protocol

Use CORD and DCP experiments to set up DCP-CORD. Set up magnet field drift compensation. Choose acquisition times in the indirect dimensions for the best resolution/sensitivity compromise.

Samples

The experiments were performed with precipitated microcrystalline u-Ubq

Processing and analysis

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Pulse Sequence and Parameter Set