HRMAS: HNCa in u-13C,15N KcsA


S. Grzesiek & A. Bax, J. Magn. Reson. 96, 432 - 440 (1992) [] J. Schleucher, M. Sattler & C. Griesinger, Angew. Chem. Int. Ed. 32, 1489-1491 (1993) L.E. Kay, G.Y. Xu & T. Yamazaki, J. Magn. Reson. A109, 129-133 (1994) [] J. Stonehouse, R.T. Clowes, G.L. Shaw, J. Keeler & E.D. Laue, J. Biomol. NMR 5, 226-232 (1995)


TROSY based HNCa decreases linewidth by x1.5 but also decreases S/N by x2 compared with regular HNCa


The experiments were performed with a precipitated 2H(Calpha) U-13C/15N KcsA, LPR =1, 9:1 DOPE-DOPS D2O citrate buffer pH 3.5

Processing and analysis

Pulse Sequence and Parameter Set