HRMAS: HNCO in u-13C,15N KcsA

Citation

S. Grzesiek & A. Bax, J. Magn. Reson. 96, 432 - 440 (1992) [https://www.sciencedirect.com/science/article/pii/002223649290099S]https://www.sciencedirect.com/science/article/pii/002223649290099S) J. Schleucher, M. Sattler & C. Griesinger, Angew. Chem. Int. Ed. 32, 1489-1491 (1993) https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.199314891 L.E. Kay, G.Y. Xu & T. Yamazaki, J. Magn. Reson. A109, 129-133 (1994) [https://www.sciencedirect.com/science/article/pii/S1064185884711454?via%3Dihub]https://www.sciencedirect.com/science/article/pii/S1064185884711454?via%3Dihub) J. Stonehouse, R.T. Clowes, G.L. Shaw, J. Keeler & E.D. Laue, J. Biomol. NMR 5, 226-232 (1995) https://link.springer.com/article/10.1007/BF00211750

Protocol

a. F2 dimension (15N) should be REVERSE. but only for HNCO not for HNCA b. This pulse sequence works better than other hnco's based on 1D optimization. c. For 3D sequences, States_TTPI works better than EA. There are no issues with T1 noise and S/N is better. d. For 2D sequences, States_TTPI has worse T1 noise.

Samples

The experiments were performed with a precipitated fractionally deuterated U-13C/15N KcsA, LPR =1, 9:1 DOPE-DOPS D2O citrate buffer pH 3.5

Processing and analysis

Pulse Sequence and Parameter Set