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to improve amide signal - use fractionally 2H (at Calpha) Kcsa at pH 3.5 8 resolved resonance at pH 6.5 and 40 resolved resonance at pH 3.5
DIPSI length: 12ms - no crosspeaks 50ms - a couple of crosspeaks 75ms - more crosspeaks.
DIPSI should be rotor synchronized (N x spinning frequency, N=1,2)
The experiments were performed with a precipitated fractionally deuterated U-13C/15N KcsA, LPR =1, 9:1 DOPE-DOPS D2O citrate buffer pH 3.5