HRMAS: 1H-15N TROSY of fractionally deuterated (mostly at Calpha) KcsA


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a. Increasing spinning frequency above 8kHz doesn't bring any improvements in most cases. b. SMSQ10.100 and SMSQ10.50 have better performance than SINE.100 and SINE.50 c. use -DLABEL_CN flag on with Crp80,0.5,20.1 pulse for 13C-15N labeled sample. The pulse refocuses 13C(O) and sharpens up 15N lines substantially


The experiments were performed with a precipitated 2H (Capha), U-13C/15N KcsA, LPR =1, 9:1 DOPE-DOPS 20% D2O citrate buffer pH 3.

Processing and analysis

Pulse Sequence and Parameter Set