Current and Upcoming Courses at CoMD/NMR and NYSBC

NMR Spectroscopy of Macromolecules

  Columbia University GG6270
  Instructors: Hashim Al-Hashim, Ann McDermott and Arthur Palmer
  Location: New York Structural Biology Center A-11
  Spring 2024
  Times: Wednesday 1:00 to 2:30 pm (lecture)
              Wednesday 2:30 to 4:00 pm (problem session)
              Thursday 1:00 to 2:30 pm (lecture

For audit/enrollment information contact Whitney Thomas

Textbook: 
       Protein NMR Spectroscopy: Principles and Practice
       Cavanagh, Fairbrother, Palmer, Rance, Skelton
       Academic Press, 2007
       ISBN 978-0-12-164491-8

Optional:
       Multidimensional Solid-State NMR and Polymers
       Schmidt-Rohr and Spiess
       Academic Press, 1996
       ISBN 0-12-626630-1

Course Description

Multi-dimensional NMR spectroscopy has become a powerful technique for investigation of the structural and dynamical properties of biological macromolecules in solution and solid state. This course will present a detailed survey of the principles of modern techniques in NMR spectroscopy as applied to biological macromolecules, including proteins, nucleic acids and carbohydrates. Both theoretical and experimental aspects of NMR spectroscopy, structure determination and investigation of rate processes will be discussed. The theoretical description of NMR spectroscopy will rely heavily on a rigorous, but simplified, quantum mechanical treatment known as the product operator formalism.

The format of the course consists of lectures by the instructors and readings from the original literature. Problem sets will be based on the lectures and reading assignments. Grading will be based on a mid-term examination, and a final paper. Paper topics will be chosen by the students in consultation with the instructors.

Students and postdoctoral scientists already performing NMR research are invited to audit any or all parts of the course. Additional Reading List

A. Abragam “Principles of Nuclear Magnetism” Claredon Press (1961).
R. R. Ernst, G Bodenhausen and A Wokaun “Principles of Nuclear Magnetic Resonance in One and Two Dimensions” Oxford Science Publications (1987).
U. Haeberlen“High Resolution NMR in Solids: Selective Averaging” (1976), also published in “Advances in Magnetic Resonance, Supplement 1”.
P. J. Hore “Nuclear Magnetic Resonance” Oxford Chemistry Primers (1995)
P. J. Hore, J. A. Jones, and S. Wimperis “NMR : The Toolkit” Oxford Chemistry Primers (2000)
G. C. K Roberts (Ed.) “NMR of Macromolecules: A practical approach” IRL Press (1993).
K. Wüthrich “NMR of Proteins and Nucleic Acids” Wiley (1986).